Specifications

IQF DiUb TechnologyLifeSensors’ diubiquitin substrates represent a new class of substrates for the continuous fluorescent measurement of true isopeptidase activity. The C-terminus of wild type ubiquitin is conjugated via an isopeptide bond to lysine 48 (K48) of a second ubiquitin molecule with the resultant diubiquitin forming an internally quenched fluorescent FRET pair (IQF). Each ubiquitin is labeled with a single molecule of either a fluorescent reporter (i.e. TAMRA) or a highly efficient quenching dye. Cleavage of the IQF DiUb by deubiquitylases leads to separation of the fluorophore from quencher and subsequent increase in observed fluorescence.

IQF DiUb VariantsThere is a remarkable difference between K48- and K63-linked ubiquitin molecules in their three-dimensional structure, which accounts for their individual functions. Beyond differential linkages (e.g. K48, K63, K11), LifeSensors has created subpanels of IQF diubiquitin substrates within each linkage.  Because each DUB is likely to recognize and cleave substrates with unique steric considerations, these subpanels vary in location of reporter fluorophore and quencher.  We recommend that each DUB be empirically evaluated against a panel of IQF DiUb substrates to select the the optimal fluorophore/quencher pairing.

IQF DiUb substrate panelsIn order to efficiently determine the optimal substrate for any DUB, or rapidly assess a panel of various DUBs, LifeSensors offers comprehensive panels for either K48 (DiUb48 panel cat no. DU0101) or K63 linked diubiquitins  (DiUb63 Panel DU0102). Each panel contain all six variants of the respective linkage along with a control enzyme (USP2core). In addition there is also a full (K48/K63) panel (DU0201) that contains all 12 variants.Need bulk quantities? We would be happy to work out a custom price that fits your budget. Please email info@lifesensors.com for more information.This product is covered by US patent # 8,518,660. By purchasing this product, the purchaser agrees to comply with the terms of our Limited Use Label License.

Info

LifeSensors成立于1996年，位于美国宾夕法尼亚州费城，是一家为药物发现与研究提供方法和可行性技术方法的生物技术公司。公司致力于为泛素和泛素蛋白酶体通路研究领域提供高品质的抗体、酶、蛋白质等试剂和试剂盒，产品最全、最新，是泛素研究者的最佳选择。其原核和真核蛋白表达系统SUMOpro和SUMOstar已被世界范围内的学术、政府研究机构和制药、生物技术公司广泛应用。通过提供创新的研究工具，LifeSensors已成为泛素和泛素样蛋白途径的领导者。