Thiskitallowsyoutodetectwhetheryourtargetproteinisubiquitylatedanddeterminethelinkagetypeoftheubiquitinchain.
Determiningthelinkageofpolyubiquitinontargetproteinsischallenging.ThetrADItionalmethodsareeitherthroughMassSpectrometrywhichiscumbersomeorimmunoblotwith linkagespecific antibodies,whichareunreliable.LifeSensorshasdevelopedamoredefinitivemethodfordemonstratingtheubiquitylationlinkageofaprotein,whichistocoupleimmunoprecipitationofpolyubiquitylatedproteinwithdigestionbya linkagespecificdeubiquitylase priortoimmunoblotanalysis. Anincreasedsignalfortheunmodifiedsubstrateoradecreasedsignalof polyubiquitylated substrateat high molecularweightafterK48/K63specificDUBtreatmentisaclearindicationthattheproteinwasK48/K63ubiquitylated. WehavebuiltthiskitaroundtheuseofMagneticTandemUbiquitinBindingEntities(TUBEs)whichbindtoallubiquitinchainlinkages.Magnetic-TUBEsareTUBEmoietiesdirectlycoupledtomagneticbeads,fortheidentificationandcharacterizationofpolyubiquitinatedproteinsbywesternblottingand/ordownstreamproteomicstudies. Magnetic-TUBEsfacilitateconvenient"one-step"pull-downofpolyubiquitinatedproteins.
ThiskitincludesthefollowingDUBs:
- ALys48linkagespecificDUB-25 µg,10 µM
- AbroadspectrumDUB-25 µg,10 µM
Applications
- Convenientone-steppull-downofpolyubiquitylatedproteinsfromcellandtissueextracts
- Isolateubiquitylatedproteinsforproteomicstudies
- Confirmationoftargetproteinubiquitylation
Benefits
- Realproofofubiquitylation–nomassspecneeded!
- Verificationthatthepatternobservedisfromubiquitin,nototherpost-translationalmodifications
- Easycomparisonofpolyubiquitinlevelsbetweentreatedsamples
- EasyscreeningformultiplePOIs
- Nosubstratepurificationneeded
- Controlforantibodynotrecognizingubiquitylatedsubstrate
- Sufficientforaminimumof10pull-downs
Documents
LifeSensors成立于1996年，位于美国宾夕法尼亚州费城，是一家为药物发现与研究提供方法和可行性技术方法的生物技术公司。公司致力于为泛素和泛素蛋白酶体通路研究领域提供高品质的抗体、酶、蛋白质等试剂和试剂盒，产品最全、最新，是泛素研究者的最佳选择。其原核和真核蛋白表达系统SUMOpro和SUMOstar已被世界范围内的学术、政府研究机构和制药、生物技术公司广泛应用。通过提供创新的研究工具，LifeSensors已成为泛素和泛素样蛋白途径的领导者。
本网站将在规定时间内给予删除等相关处理。
