![LifeSensors/NS105-10mg Ubiquilabel(TM) - [C-13(99%), N-15(98%), H-2(50%)]/NS105-10mg Ubiquilabel(TM) - [C-13(99%), N-15(98%),](images/lifesensors/7 - Ubiquitin and Derivatives white background canva-350x350.png)
13C, 15N and 2H, heavy-labeled ubiquitin provides a stable standard for ubiquitin structural study by NMR. The recombinant human ubiquitin is purified from E.coli expressing the human ubiquitin gene. The cells are grown in media where the sole nitrogen source is 15N-labeled ammonium sulfate, the sole carbon source is 13C-glucose, and respective amount of 2H is supplied where applicable. The recombinant human ubiquitin is purified without heating by a proprietary procedure that preserves the native structure of the protein. The purified protein is homogenous when analyzed by overloaded SDS gels. The pools of purified protein solution are extensively dialyzed against water and lyophilized.
Info
Purity | 98% by RP-HPLC |
Protein | Ubiquitin |
Atom % Enrichment | 13C (99%), 15N (98%), 2H (50%) |
Physical state | Lyophilized powder |
Quantity | 10 mg |
Solubility | >30 mg/mL in aqueous solution |
Storage | -20°C |
Applications
- Structural study of Ub by NMR
- Benefits: >98% isotope enrichment
Documents
LifeSensors成立于1996年，位于美国宾夕法尼亚州费城，是一家为药物发现与研究提供方法和可行性技术方法的生物技术公司。公司致力于为泛素和泛素蛋白酶体通路研究领域提供高品质的抗体、酶、蛋白质等试剂和试剂盒，产品最全、最新，是泛素研究者的最佳选择。其原核和真核蛋白表达系统SUMOpro和SUMOstar已被世界范围内的学术、政府研究机构和制药、生物技术公司广泛应用。通过提供创新的研究工具，LifeSensors已成为泛素和泛素样蛋白途径的领导者。
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