UBE2M (also known as Ubc12) is an E2 conjugation enzyme that is involved in the ubiquitylation of target substrates, along with E1 and E3 enzymes. UBE2M (Ubc12) is an E2 that is involved in the conjugation of the ubiquitin-like protein (Ubl) NEDD8 to target substrates (NEDDylation) along with the APPBP1/Uba3 activating enzyme. NEDDylation is required for cell cycle progression. In addition, NEDD8 is conjugated to cullin family members which are a part of SCF ubiquitin E3 ligase complexes, thereby activating them.
Info
| Amount | 0.8 nmol |
| State | Liquid |
| Molecular Weight | 21,894 Da |
| Source | Human Recombinant |
| Fusion Tag | His6 |
| Accession Number | P61081 |
| Alternate Names | Ubc12 |
| Storage | -80°C, avoid freeze/thaw cycles |
| Stability | 1 year under recommended storage conditions |
| Recommended Use | Enzyme concentration of 100 nM – 1 mM is recommended for in vitro conjugation. |
Applications
- Identify cognate E2/E3 pairs
- Characterize upregulation/downregulation of E2 enzymes
- Build poly-ubiquitin chains
- Characterize ubiquitin chain specificity of E2 enzymes
- Analyze expression of E2 enzymes
Benefits
- Untagged (native) E2 conjugation enzyme
Documents
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