Poly-ubiquitylationoftargetproteinsthroughlinkageatK48,isnowthemostthoroughlystudiedofthevariouschainlinkages,andweonceconsideredthehallmarkofthispost-translationalmodification.Itisnowclearthatmany,ifnotall,poly-Ubchaintopologieslikelyplaydistinctandimportantrolesinregulatingcellularprocesses.Nevertheless,K48linkageremainsacriticalpathwayforthecellstomaintainhomeostasisthroughproteolyticdegradation,andassuchremainsveryintriguingforthestudyofDUBsthatplayaroleinthedegradation,aswellastheproteasomeitself.
Thesetri-ubiquitinchainsgeneratedfromtheenzymaticlinkageofwild-typeubiquitinthroughlysine48.Themostdistalubiquitincontainsanargininesubstitutionforthelysineatposition48,limitingchainlength.
Info
| Source | Humanrecombinant |
| Form | Liquid |
| MolecularWeight | 25,686.5Da |
| AffinityTag | None |
| Concentration | Lotspecific |
| Buffer | 20mMTris,pH7.5,0.15MNaCl,1mMEDTA |
| Storage | -80°C,avoidcyclesoffreeze/thaw |
| StABIlity | 1yearunderrecommendedstorageconditions |
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