Specifications

TUBEs: ARevolutioninPolyubiquitinIsolationandCharacterization

TheuseofTandemUbiquitinBindingEntities(TUBEs)isemergingasanindispensablestrategyforubiquitinresearch.ThefirstgenerationoftheseTUBEsbindK48-andK63-linkedtetraUbchainswithsingledigitnanomolarKds,~100to1000-foldmoretightlythanmonomericUBAs.TUBEsalsoprotectproteinsfromDUBsandtheproteasome,evenintheabsenceofinhibitorsnormallyrequiredtoblocksuchactivity.ThisallowsefficientisolationofnativepolyUbchainsandattachedproteinsfromcelllines,tissues,andorgansunderconditionsthatarelesslikelytoaltercellphysiologythanthoselistedabove.TUBE1andTUBE2haverecentlybeendemonstratedtoenrichforallpolyUbchainlinkagetypes,withoutdiscrimination,makingthesereagentsappropriateevenifthelinkagetypeisnotknownfortheproteinofinterest.

ThenextgenerationofTUBEs: LinkageSpecificIsolationofPolyUbChains

K48TUBEHFwasdevelopedtoshowenhancedselectivityforK48-linkedpolyubiquitinchains(~20nM)overallotherlinkages(>2µM).ItcanbeusedaloneorinconjunctionwithourotherTUBEproducts,especiallyK63TUBEandM1(linear)TUBEtoinvestigatepolyubiquitinchainlinkageinyoursubstrateprotein.

Note:ThenewK48TUBEHFhasacomparableaffinityasourexistingK63TUBE.BothofthembindtotheircognateUb4swithaKdof~10-20nM.

Magnetic-TUBEsareTUBEmoietiesdirectlycoupledtomagneticbeads,fortheidentificationandcharacterizationofpolyubiquitinatedproteinsbywesternblottingand/ordownstreamproteomicstudies.Magnetic-TUBEsfacilitateconvenient“one-step”pull-downofpolyubiquitinatedproteins.

Info

K48TUBEHF,MagneticSize:1mlBuffer:PBS,pH7.2,20%ethanolStorage:Stableforupto6monthsat4oC.Donotcentrifugeathighspeeds(>3000g),dryorfreezethebeads.

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